mouse anti rat proliferating cell nuclear antigen pcna  (Boster Bio)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAD and further refeeding with a VAS diet cause changes in cell proliferation in the mammary gland. (A) Immunohistochemistry for PCNA in the mammary gland. The black arrows indicate positive PCNA + cells. Magnification 100×. The scale bar represents 25 μm. (B) Quantification of the percentage of PCNA + cells from the immunohistochemistry images shown in (A). Data are shown as representative images or mean values ± SDs ( n = 10).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: Immunohistochemistry

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAS and further refeeding with a VAS diet cause changes in apoptosis and proliferation in the mammary gland. Determination of the TUNEL + /PCNA + cell ratios from the immunohistochemistry images shown in Figures A and A. The data are presented as the means ± SDs ( n = 4).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: TUNEL Assay, Immunohistochemistry

Journal: Plastic & Reconstructive Surgery

Article Title: Tracing the Change and Contribution of Subcutaneous Adipose to Skin Expansion Using a Luciferase-Transgenic Fat Transplantation Model

doi: 10.1097/prs.0000000000010753

Figure Lengend Snippet: Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and PCNA to mark ves- sels and proliferating cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.

Article Snippet: To detect the vessels and proliferating cells (early G1 and S phases) in expanded skin, rabbit anti-rat CD31 (BM2966; Boster, Wuhan, People’s Republic of China) and mouse anti-rat proliferating cell nuclear antigen (PCNA) (BM0104; Boster) antibodies were applied.

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Western Blot

Journal: PLoS ONE

Article Title: Chronic VEGF Blockade Worsens Glomerular Injury in the Remnant Kidney Model

doi: 10.1371/journal.pone.0039580

Figure Lengend Snippet: A) Fractional cortical interstitial area; B) Cortical interstitial infiltration by macrophages; C) Tubular cells in proliferation (PCNA + ); D) Interstitial cells in proliferation (PCNA + ); a p<0.05 vs. respective S; b p<0.05 vs. respective untreated; c p<0.05 vs. respective value on Day 7.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-rat ED-1 antibody (for macrophage detection; Serotec, Oxford, United Kingdom); monoclonal mouse anti-rat proliferating cell nuclear antigen (PCNA) (Dako, Glostrup, Denmark); monoclonal mouse anti-rat endothelial aminopeptidase P (JG-12) (Bender MedSystems, California, USA); polyclonal rabbit anti-human zonula occludens-1 (ZO-1) (Zymed, San Francisco, USA); and monoclonal mouse anti-human Wilms’ tumor 1 (WT-1) (Dako, Glostrup, Denmark).

Techniques:

Journal: Oncology Letters

Article Title: Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice

doi: 10.3892/ol.2021.12999

Figure Lengend Snippet: (A) Mammary carcinomas induced in the DMBA-treated heterozygous Trp53 knockout mice exhibited highly differentiated glandular structures (H&E staining; scale bar, 50 µm), and (B) featured a characteristic biphasic structure consisting of two differentiation types of luminal (red; cytokeratin 18) and myoepithelial cells (brown; αSMA); the lower right box is a higher magnification image of the lower middle box. (C) H&E staining; scale bar, 20 µm. (D) Immunohistochemistry for estrogen receptor α revealed N positivity in the inner carcinoma cells; the middle left box is a higher magnification image of the middle right box. (E) N positivity for pERK was primarily present in inner cells. (F) M positivity for β-catenin was weak in outer cells (arrows); the upper left box is a higher magnification image of the upper left box. (G) pAKT was negative. (H) Proliferating cell nuclear antigen positivity was primarily present in outer cells; the upper box is a higher magnification image of the middle right box. (I) N positivity for dual-specificity phosphatase was present in both inner and outer cells. (J) Summary of immunohistochemical analysis of mammary carcinomas in DMBA-treated Trp53 heterozygous knockout mice. DMBA, 7,12-dimethylbenz[a]anthracene; N, nuclear; M, membranous. H&E, hematoxylin and eosin; αSMA, α-smooth muscle actin.

Article Snippet: Then, the sections were incubated with 3% hydrogen peroxide (at room temperature for 10 min), 10% normal goat serum (at room temperature for 20 min.; Nichirei Biosciences, Inc.), and with the following primary antibodies 4°C overnight: Anti-human ERα mouse monoclonal (1:50 dilution; clone 6F11; cat. no. NCL-ER-6F11; Novocastra), anti-human/mouse phosphorylated-ERK1(pERK1; T202/Y204)/ERK2 (pERK2; T185/Y187) rabbit polyclonal (1:500 dilution; cat. no. AF1018; R&D Systems, Inc.), anti-human dual-specificity phosphatase (DUSP)4 rabbit polyclonal (1:50 dilution, cat. no. ab72593; Abcam) anti-human phosphorylated-AKT (pAKT; Ser473) rabbit monoclonal (1:100 dilution; clone D9E; cat. no. #4060; Cell Signaling Technology, Inc.), anti-mouse β-catenin mouse monoclonal (1:500 dilution; clone 14; cat. no. 610154; BD Transduction Laboratories), anti-human cytokeratin 18 rabbit polyclonal (1:1,000 dilution; cat. no. 10830-1-AP; ProteinTech Group, Inc.), anti-human α-smooth muscle actin (αSMA) rabbit monoclonal (1:1,000 dilution; clone EPR5368; cat. no. ab124964; Abcam) and anti-rat proliferating cell nuclear antigen (PCNA) mouse monoclonal (1:800 dilution; clone PC10; cat. no. M0879; DakoCytomation).

Techniques: Knock-Out, Staining, Immunohistochemistry, Immunohistochemical staining

Journal: Oncology Letters

Article Title: Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice

doi: 10.3892/ol.2021.12999

Figure Lengend Snippet: (A) Mammary carcinomas induced in PhIP-treated Trp53 heterozygous knockout mice demonstrated moderately differentiated solid/microacinar structures (H&E staining; scale bar, 50 µm). (B) Myoepithelial cells positive for αSMA (brown) were scattered among luminal cells; the lower right box is a higher magnification image of the lower right box. (C) Microacinar type with small ductules. H&E staining; scale bar, 20 µm. (D) Immunohistochemistry for estrogen receptor α was negative in 5/10 cases. (E) Solid type. H&E staining; scale bar, 20 µm. (F) CN positivity for pERK was detected in the peripheral area of the nodules. (G) CN positivity for β-catenin was detected; the lower right box is a higher magnification image of the upper left box. (H) M positivity for pAKT was diffusely scattered. The box in the upper right is a higher magnification image of the lower left box. (I) Proliferating cell nuclear antigen is diffusely positive. (J) N positivity for dual-specificity phosphatase is weak. (K) Summary of immunohistochemical analysis of mammary carcinomas in PhIP-treated Trp53 heterozygous knockout mice. PhIP, 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; CN, cytoplasmic and nuclear; N, nuclear; M, membranous; H&E, hematoxylin and eosin; αSMA, α-smooth muscle actin.

Article Snippet: Then, the sections were incubated with 3% hydrogen peroxide (at room temperature for 10 min), 10% normal goat serum (at room temperature for 20 min.; Nichirei Biosciences, Inc.), and with the following primary antibodies 4°C overnight: Anti-human ERα mouse monoclonal (1:50 dilution; clone 6F11; cat. no. NCL-ER-6F11; Novocastra), anti-human/mouse phosphorylated-ERK1(pERK1; T202/Y204)/ERK2 (pERK2; T185/Y187) rabbit polyclonal (1:500 dilution; cat. no. AF1018; R&D Systems, Inc.), anti-human dual-specificity phosphatase (DUSP)4 rabbit polyclonal (1:50 dilution, cat. no. ab72593; Abcam) anti-human phosphorylated-AKT (pAKT; Ser473) rabbit monoclonal (1:100 dilution; clone D9E; cat. no. #4060; Cell Signaling Technology, Inc.), anti-mouse β-catenin mouse monoclonal (1:500 dilution; clone 14; cat. no. 610154; BD Transduction Laboratories), anti-human cytokeratin 18 rabbit polyclonal (1:1,000 dilution; cat. no. 10830-1-AP; ProteinTech Group, Inc.), anti-human α-smooth muscle actin (αSMA) rabbit monoclonal (1:1,000 dilution; clone EPR5368; cat. no. ab124964; Abcam) and anti-rat proliferating cell nuclear antigen (PCNA) mouse monoclonal (1:800 dilution; clone PC10; cat. no. M0879; DakoCytomation).

Techniques: Knock-Out, Staining, Immunohistochemistry, Immunohistochemical staining